Hemoglobin Check Using Sahli’s Method The Sahli's method, also known as the acid hematin method, is a manual technique to estimate hemoglobin concentration in the blood. Here is a step-by-step guide to performing a hemoglobin check using Sahli's method: Fig. 1 Sahil's Heamoglobinometer Materials Needed: 1. Sahli's hemoglobinometer (also called a hemoglobinometer or hemometer) 2. Sahli's pipette (0.02 ml capacity) 3. Sahli’s graduated hemoglobin tube 4. N/10 hydrochloric acid (HCl) 5. Distilled water 6. Mixing rod (usually a glass rod) 7. Blood sample (finger prick or venous blood) 8. Lancet and alcohol swabs (if using a finger prick) 9. Cotton balls or gauze Procedure: 1. Preparation : - Ensure all equipment is clean and dry. - Place the Sahli’s hemoglobinometer on a flat, stable surface. 2. Filling the Tube : - Add N/10 hydrochloric acid (HCl) to the graduated hemoglobin tube up to the 2 g/dl mark. This creates a starting point for the acid hemati...
Everything Easy